Torsteinsdóttir S, Georgsson G, Gísladóttir E, Rafnar B, Pálsson P A, Pétursson G
Institute for Experimental Pathology, University of Iceland, Keldur, Reykjavík.
J Neuroimmunol. 1992 Dec;41(2):149-58. doi: 10.1016/0165-5728(92)90065-s.
There are several indications that central nervous system (CNS) lesions in visna are immune-mediated and that cell-mediated immunity (CMI) may be of importance in the initiation of the lesions. To study the role of CMI in the pathogenesis of CNS lesions, five sheep were infected by intracerebral inoculation with visna virus and observed for 1 year. The following parameters were monitored at regular intervals: (1) neutralizing and ELISA antibodies; (2) visna virus-specific stimulation of lymphocytes from peripheral blood; (3) lymphocyte subpopulations in peripheral blood, cerebrospinal fluid (CSF) and brain at sacrifice. The CNS lesions were graded and compared with other parameters. The time course and titers of antibodies did not correlate with the severity of CNS lesions whereas the CMI did, indicating that CMI may play an important role in lesion development. The correlation of the number of CD8-positive cells in the CSF with the severity of lesions and the reversed ratio of CD4/CD8-positive cells in the diffusely infiltrated neuroparenchyma indicates that the CD8-positive T lymphocyte may be an important effector cell in the induction of CNS lesions.
有若干迹象表明，维斯纳病中的中枢神经系统（CNS）病变是免疫介导的，并且细胞介导免疫（CMI）可能在病变的起始过程中起重要作用。为了研究CMI在CNS病变发病机制中的作用，对5只绵羊进行脑内接种维斯纳病毒感染，并观察1年。定期监测以下参数：（1）中和抗体和ELISA抗体；（2）外周血淋巴细胞对维斯纳病毒的特异性刺激；（3）处死时外周血、脑脊液（CSF）和脑内的淋巴细胞亚群。对CNS病变进行分级，并与其他参数进行比较。抗体的时间进程和滴度与CNS病变的严重程度无关，而CMI与之相关，这表明CMI可能在病变发展中起重要作用。CSF中CD8阳性细胞数量与病变严重程度的相关性以及弥漫性浸润神经实质中CD4/CD8阳性细胞比例的倒置表明，CD8阳性T淋巴细胞可能是诱导CNS病变的重要效应细胞。
