Onisk D V, Donis R, Kelling C L, Srikumaran S
Department of Veterinary Science, University of Nebraska, Lincoln.
Viral Immunol. 1992 Winter;5(4):257-63. doi: 10.1089/vim.1992.5.257.
Two murine neutralizing monoclonal antibodies (MAbs), 4D8 and 6D11, recognizing epitopes on gp53, a surface glycoprotein of bovine viral diarrhea virus (BVDV), were used to generate anti-idiotypic antibodies (anti-ids) in a calf. The polyclonal anti-ids were isolated from serum by affinity chromatography on their respective Ab-1-Sepharose columns, followed by repeated adsorption on isotype-matched antibody-Sepharose columns. The anti-ids reacted specifically with their respective Ab-1, but not with isotype-matched controls. They also inhibited the binding of their Ab-1 to BVDV in a concentration-dependent manner. Mice immunized with the two anti-id preparations developed antibodies to BVDV, which neutralized the virus in vitro.
两种识别牛病毒性腹泻病毒（BVDV）表面糖蛋白gp53上抗原表位的鼠源中和单克隆抗体（MAb）4D8和6D11，被用于在一头小牛体内产生抗独特型抗体（抗-ids）。通过在各自的Ab-1-琼脂糖柱上进行亲和层析，随后在同型匹配抗体-琼脂糖柱上反复吸附，从血清中分离出多克隆抗-ids。抗-ids与各自的Ab-1特异性反应，但不与同型匹配对照反应。它们还以浓度依赖的方式抑制其Ab-1与BVDV的结合。用两种抗-独特型制剂免疫的小鼠产生了针对BVDV的抗体，这些抗体在体外中和了该病毒。
