Xue W, Minocha H C
Department of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
J Gen Virol. 1993 Jan;74 ( Pt 1):73-9. doi: 10.1099/0022-1317-74-1-73.
We have produced and characterized polyclonal anti-idiotypic antibodies (anti-ids) that mimic the antigenic structures of gp53 from bovine viral diarrhoea virus (BVDV). In this study, the anti-ids were used to identify cell receptors for BVDV. The anti-ids bound specifically to bovine cells, as determined by flow cytometric analysis, and inhibitory binding assays showed that they bound to the cell surface receptors for BVDV. A cell surface protein with an M(r) of approximately 50K was immunoprecipitated by the anti-ids from MDBK cells; this was blocked by preincubation of cell lysate with BVDV. This indicates that the 50K protein might be a specific receptor for BVDV gp53. Thirteen BVDV strains were used to evaluate inhibition of anti-id binding to MDBK cells and inhibition of BVDV infection of anti-id-treated MDBK cell monolayers. Results demonstrated that both processes were inhibited to varying degrees depending on virus strain. The results suggested that multiple receptors for BVDV attachment may exist on MDBK cells, and that different virus strains do not have the same receptor.
我们制备并鉴定了模拟牛病毒性腹泻病毒（BVDV）gp53抗原结构的多克隆抗独特型抗体（抗独特型抗体）。在本研究中，这些抗独特型抗体被用于鉴定BVDV的细胞受体。通过流式细胞术分析确定，抗独特型抗体与牛细胞特异性结合，抑制性结合试验表明它们与BVDV的细胞表面受体结合。抗独特型抗体从MDBK细胞中免疫沉淀出一种分子量约为50K的细胞表面蛋白；细胞裂解物与BVDV预孵育可阻断这种沉淀。这表明50K蛋白可能是BVDV gp53的特异性受体。使用13株BVDV评估抗独特型抗体与MDBK细胞结合的抑制情况以及抗独特型抗体处理的MDBK细胞单层中BVDV感染的抑制情况。结果表明，这两个过程均根据病毒株的不同而受到不同程度的抑制。结果提示，MDBK细胞上可能存在多种BVDV附着受体，并且不同病毒株的受体不同。
