Curry S, Abu-Ghazaleh R, Blakemore W, Fry E, Jackson T, King A, Lea S, Logan D, Newman J, Stuart D
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.
J Mol Biol. 1992 Dec 20;228(4):1263-8. doi: 10.1016/0022-2836(92)90332-e.
Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A10(61), A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10(61). In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2SO4 or mixtures of polyethylene glycol and NH4Cl.
O、A和C型口蹄疫病毒已被结晶。所研究的特定毒株包括O1K、A10(61)、A22伊拉克24/64、A24克鲁塞罗和C-S8c1。此外，还培养出了G67（一种源自O1K的单克隆抗体中和逃逸突变体）以及在用C-S8c1在幼仓鼠肾细胞中建立持续感染后回收的病毒R100的晶体。空颗粒，即缺乏RNA基因组的衣壳，也已针对A22伊拉克24/64和A10(61)亚型进行了结晶。几乎在所有情况下，适合进行高分辨率结构测定的晶体都是从硫酸铵或聚乙二醇与氯化铵的混合物中获得的。
