低浓度戊二醛对1型脊髓灰质炎病毒和25型艾柯病毒衣壳蛋白的活性
Activity of glutaraldehyde at low concentrations against capsid proteins of poliovirus type 1 and echovirus type 25.
作者信息
Chambon M, Bailly J L, Peigue-Lafeuille H
机构信息
Laboratoire de Bactériologie-Virologie, Faculté de Médecine et Pharmacie, Clermont-Ferrand, France.
出版信息
Appl Environ Microbiol. 1992 Nov;58(11):3517-21. doi: 10.1128/aem.58.11.3517-3521.1992.
The activity of glutaraldehyde (GTA) against capsid proteins of poliovirus type 1 and echovirus type 25 was studied to understand the mode of action of this reagent against enteroviruses. The viruses were treated with GTA concentrations ranging from 0.005 to 0.10%. In the poliovirus particles, high-molecular-weight products were formed by 0.05% GTA, whereas in the echovirus particles, they were formed at 0.005% GTA. These products consist of complexes composed essentially of VP1 and VP3. There seemed to be differences in the composition of the complexes in the two viruses. Cross-linkings between the two polypeptides of the poliovirus capsid may be due to the accessibility to GTA of lysine residues on the loops of VP1 and VP3, which twist out from the surface of the shell.
为了解戊二醛（GTA）对肠道病毒的作用模式，研究了其对1型脊髓灰质炎病毒和25型埃可病毒衣壳蛋白的活性。用浓度范围为0.005%至0.10%的GTA处理病毒。在脊髓灰质炎病毒颗粒中，0.05%的GTA形成了高分子量产物，而在埃可病毒颗粒中，0.005%的GTA就形成了这些产物。这些产物由主要由VP1和VP3组成的复合物构成。两种病毒中复合物的组成似乎存在差异。脊髓灰质炎病毒衣壳两种多肽之间的交联可能是由于VP1和VP3环上赖氨酸残基可被GTA接近，这些环从衣壳表面扭曲出来。
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