Beard C W, Schnitzlein W M, Tripathy D N
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Avian Dis. 1992 Oct-Dec;36(4):1052-5.
A recombinant fowlpox vaccine virus containing the H5 hemagglutinin gene of avian influenza virus was administered to susceptible chickens via wing-web puncture, eye drop, instillation into the nares, and drinking water. Even though there was a negligible hemagglutination-inhibition (HI) serologic response, all 10 chickens vaccinated by wing-web puncture remained without obvious signs of disease and survived challenge with a highly pathogenic strain of H5N2 avian influenza virus. All unvaccinated chickens and those vaccinated by nasal and drinking-water routes died following challenge. Eight of 10 chickens vaccinated with the recombinant by eyedrop died. All vaccinates were negative on the agar gel precipitin (AGP) test, and only one chicken had a positive HI titer before challenge. All chickens that survived challenge had high levels of HI antibody and were positive on the AGP test, indicating that they were infected by the challenge virus.
一种含有禽流感病毒H5血凝素基因的重组鸡痘疫苗病毒，通过翼膜穿刺、滴眼、滴鼻和饮水的方式接种给易感鸡。尽管血凝抑制（HI）血清学反应微乎其微，但通过翼膜穿刺接种的10只鸡均未出现明显的疾病迹象，并在感染高致病性H5N2禽流感病毒后存活下来。所有未接种疫苗的鸡以及通过鼻腔和饮水途径接种疫苗的鸡在感染后均死亡。通过滴眼接种重组疫苗的10只鸡中有8只死亡。所有接种疫苗的鸡在琼脂凝胶沉淀素（AGP）试验中均为阴性，只有一只鸡在感染前HI效价呈阳性。所有在感染后存活下来的鸡都具有高水平的HI抗体，并且在AGP试验中呈阳性，这表明它们已被感染病毒感染。
