Karaca K, Naqi S, Gelb J
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Avian Dis. 1992 Oct-Dec;36(4):903-15.
Three panels of monoclonal antibodies (MAbs) were prepared against the spike (S) proteins of infectious bronchitis virus (IBV) strains Arkansas 99, Connecticut 46, and Massachusetts 41. Based on enzyme-linked immunosorbent assay (ELISA), the MAbs were grouped into three categories: 1) group-specific, which reacted with a broad spectrum of homologous and heterologous IBV serotypes; 2) serotype-specific, which reacted only with strains of the homologous serotype; and 3) strain-specific, which reacted "selectively" with only certain strains of homologous and heterologous serotypes. MAbs that displayed serotype specificity were all specific to S1 fractions of the homologous serotype, confirming that epitopes that determine virus serotype are associated with the S1 protein. An excellent correlation was found when the results of IBV serotyping by MAb-based indirect ELISA were compared with those from the conventional virus-neutralization test. This confirms that the MAbs described here will serve as valuable tools in epizootiological studies and serotype-specific diagnosis of IBV infection.
制备了三组针对传染性支气管炎病毒（IBV）阿肯色99株、康涅狄格46株和马萨诸塞41株的刺突（S）蛋白的单克隆抗体（MAb）。基于酶联免疫吸附测定（ELISA），这些单克隆抗体被分为三类：1）组特异性，可与广泛的同源和异源IBV血清型发生反应；2）血清型特异性，仅与同源血清型的毒株发生反应；3）毒株特异性，仅“选择性”地与某些同源和异源血清型的毒株发生反应。表现出血清型特异性的单克隆抗体均对同源血清型的S1片段具有特异性，证实决定病毒血清型的表位与S1蛋白相关。当将基于单克隆抗体的间接ELISA进行IBV血清分型的结果与传统病毒中和试验的结果进行比较时，发现两者具有极好的相关性。这证实了此处所述的单克隆抗体将成为IBV感染的流行病学研究和血清型特异性诊断中的有价值工具。
