Wainright P O, Villegas P, Brugh M, Lukert P D
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Avian Dis. 1989 Jul-Sep;33(3):482-90.
Three monoclonal antibodies (MABs) reactive against two structural proteins--the nucleoprotein (NP) or the surface (S) protein--of avian infectious bronchitis virus (IBV) were produced and characterized. The MABs did not neutralize virus infectivity or inhibit hemagglutination. Their reactivity patterns with the homologous strain and eight heterologous strains of IBV were determined using the indirect immunoperoxidase test, the indirect immunofluorescent test, transfer-immunoblotting of separated proteins, and a dot-immunoblotting assay (DIA). Two MABs, NP- or S-protein-specific, reacted with all nine strains; one (NP-specific) reacted with only two strains. The two MABs reacting with all nine strains of IBV also detected 18 IBV field isolates of unknown serotype in the DIA. The MAB detecting only two strains did not react in the DIA. The diagnostic application of these MABs appears promising.
制备并鉴定了三种针对禽传染性支气管炎病毒（IBV）两种结构蛋白——核蛋白（NP）或表面（S）蛋白的单克隆抗体（MABs）。这些单克隆抗体不能中和病毒感染性或抑制血凝反应。使用间接免疫过氧化物酶试验、间接免疫荧光试验、分离蛋白的转印免疫印迹和斑点免疫印迹分析（DIA）确定了它们与IBV同源株和八个异源株的反应模式。两种分别针对NP蛋白或S蛋白的单克隆抗体与所有九个毒株都发生反应；一种（针对NP蛋白）仅与两个毒株发生反应。在DIA中，这两种与所有九个IBV毒株都发生反应的单克隆抗体还检测到18株血清型未知的IBV田间分离株。仅检测到两个毒株的单克隆抗体在DIA中不发生反应。这些单克隆抗体的诊断应用前景广阔。
