Karaca K, Naqi S
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Vet Microbiol. 1993 Mar;34(3):249-57. doi: 10.1016/0378-1135(93)90015-y.
A monoclonal antibody (Mab) blocking enzyme-linked immunosorbent assay (B-ELISA) was developed and compared to a conventional indirect ELISA (I-ELISA) and a virus-neutralization (VN) test for detection of specific antibodies to avian infectious bronchitis virus (IBV) serotypes. Sera used in this study were derived from chickens experimentally inoculated with the three most prevalent IBV serotypes, Arkansas (Ark), Connecticut (Conn), and Massachusetts (Mass). Overall, there was good correlation between the results of B-ELISA and the VN test. Both detected serotype-specific antibodies in chicken sera during primary and secondary phases of the immune response. Results of both tests indicated that the antibodies produced during the primary response to IBV serotypes are strongly serotype-specific. Those produced during the secondary response react more strongly with the homologous virus, but do exhibit some level of cross-reactivity with heterologous antigens. I-ELISA detected IBV group-specific and not serotype-specific antibodies. The B-ELISA which both offers the convenience of the conventional I-ELISA and the serotype specificity of the VN test, hold excellent promise for field application in IBV diagnosis and evaluation of response to IBV vaccines.
开发了一种单克隆抗体阻断酶联免疫吸附测定法（B-ELISA），并将其与传统间接酶联免疫吸附测定法（I-ELISA）和病毒中和试验（VN）进行比较，用于检测针对禽传染性支气管炎病毒（IBV）血清型的特异性抗体。本研究中使用的血清来自经三种最常见的IBV血清型，即阿肯色型（Ark）、康涅狄格型（Conn）和马萨诸塞型（Mass）实验接种的鸡。总体而言，B-ELISA结果与VN试验结果之间具有良好的相关性。两者在免疫反应的初级和次级阶段均检测到鸡血清中的血清型特异性抗体。两项试验结果均表明，对IBV血清型初次反应期间产生的抗体具有很强的血清型特异性。二次反应期间产生的抗体与同源病毒反应更强，但确实与异源抗原表现出一定程度的交叉反应。I-ELISA检测到的是IBV组特异性而非血清型特异性抗体。B-ELISA既具有传统I-ELISA的便利性，又具有VN试验的血清型特异性，在IBV诊断和评估对IBV疫苗的反应方面具有很好的现场应用前景。
