HERRMANN H, KLEIN N W, ALBERS G
J Cell Biol. 1964 Aug;22(2):391-401. doi: 10.1083/jcb.22.2.391.
The degree of histological deterioration of the original explant and the extent of cell spreading was evaluated in cultures of pectoralis muscle from 11-day chicks. Although the frequencies of these two parameters varied with the amounts of horse serum and embryo extract added to the medium, cultures from dystrophic chicks, in comparison to those from either normal or heterozygous animals, consistently showed the largest number of explants with the most extreme forms of histological deterioration and cell spreading. At 20 per cent horse serum the cultures from heterozygous chicks showed greater frequencies of the more extensive forms of deterioration and spreading than the normal muscle explants, but at 5 per cent horse serum these two groups appeared similar. Regardless of genetic background, cultures of the pectoralis muscle from 18-day embryos and of the latissimus dorsi muscle from 11-day chicks exhibited comparable high frequencies for the maximal degrees of deterioration and spreading.
在11日龄雏鸡胸肌培养物中评估了原代外植体的组织学恶化程度和细胞铺展程度。尽管这两个参数的频率随添加到培养基中的马血清和胚胎提取物的量而变化，但与来自正常或杂合动物的培养物相比，营养不良雏鸡的培养物始终显示出具有最极端组织学恶化和细胞铺展形式的外植体数量最多。在含20%马血清的情况下，杂合雏鸡的培养物比正常肌肉外植体显示出更广泛的恶化和铺展形式的频率更高，但在含5%马血清时，这两组看起来相似。无论遗传背景如何，18日龄胚胎胸肌培养物和11日龄雏鸡背阔肌培养物在最大程度的恶化和铺展方面都表现出相当高的频率。
