Gollard R P, Slavkin H C, Snead M L
University of Southern California, Los Angeles 23146.
Oral Surg Oral Med Oral Pathol. 1992 Dec;74(6):761-7. doi: 10.1016/0030-4220(92)90405-f.
Neonatal mouse pups were injected subcutaneously with polyoma virus to induce odontogenic tumors. This treatment resulted in a spectrum of tumors that arose in organs dependent upon epithelial-mesenchymal interactions for their organogenesis, which included the teeth, salivary glands, thymus, and lacrimal glands. In addition, several odontogenic tumors with a histologic resemblance to ameloblastoma were identified and analyzed with respect to the presence of markers specific for various stages of ameloblast differentiation. Immunodetection analyses of the odontogenic tumors identified fibronectin and laminin, typical of basement membrane organization during early tooth organogenesis. These same tumors failed to express amelogenin, a gene whose expression is limited to differentiated ameloblasts. In contrast, a 47 kDa enamelin-like polypeptide was identified with the use of an antienamelin antibody. These data were interpreted to suggest that the polyoma virus truncated the differentiation pathway for these odontogenic tissues at an early stage of their development and retained the expression of basement membrane components and the enamelin-like polypeptides, yet excluded expression of amelogenin gene products. This observation suggests that polyoma viral transformation may dysregulate odontogenic tissue interactions and produce tumors composed of cells arrested at a specific stage in their development.
将多瘤病毒皮下注射到新生小鼠幼崽体内以诱导牙源性肿瘤。这种治疗导致了一系列肿瘤的产生，这些肿瘤出现在其器官发生依赖上皮-间充质相互作用的器官中，包括牙齿、唾液腺、胸腺和泪腺。此外，还鉴定出了几种组织学上与成釉细胞瘤相似的牙源性肿瘤，并针对成釉细胞分化不同阶段的特异性标志物的存在进行了分析。对牙源性肿瘤的免疫检测分析确定了纤连蛋白和层粘连蛋白，这是早期牙齿器官发生过程中基底膜组织的典型特征。这些相同的肿瘤未能表达釉原蛋白，该基因的表达仅限于分化的成釉细胞。相比之下，使用抗釉蛋白抗体鉴定出了一种47 kDa的类釉蛋白多肽。这些数据被解释为表明多瘤病毒在这些牙源性组织发育的早期阶段截断了其分化途径，保留了基底膜成分和类釉蛋白多肽的表达，但排除了釉原蛋白基因产物的表达。这一观察结果表明，多瘤病毒转化可能会失调牙源性组织相互作用，并产生由发育停滞在特定阶段的细胞组成的肿瘤。
