Shiu S Y, Reid H W, Gould E A
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Virus Res. 1992 Dec;26(3):213-29. doi: 10.1016/0168-1702(92)90014-z.
We have constructed recombinant baculoviruses and vaccinia viruses containing cloned DNA, encoding either the envelope protein alone or all of the structural proteins (core, membrane and envelope) of louping ill virus. Glycosylated viral envelope protein, presented both inside and on the surface of insect and mammalian cells, was expressed by all four recombinant viruses. Differences in antigenic presentation of the envelope protein were observed between the envelope protein and structural protein constructs as well as between the insect and mammalian cell expression systems. Despite the expression of epitopes known to elicit neutralizing and protective antibodies when present in authentic antigen, the recombinant envelope protein expressed by either vector failed to induce, in mice or rabbits, either neutralizing or protective antibodies against louping ill virus.
我们构建了重组杆状病毒和痘苗病毒，这些病毒含有克隆的DNA，其编码的要么是单独的包膜蛋白，要么是跳跃病病毒的所有结构蛋白（核心、膜和包膜）。所有四种重组病毒均表达了糖基化的病毒包膜蛋白，该蛋白在昆虫细胞和哺乳动物细胞内部及表面均有呈现。在包膜蛋白构建体与结构蛋白构建体之间以及昆虫细胞和哺乳动物细胞表达系统之间，观察到了包膜蛋白抗原呈递的差异。尽管在真实抗原中存在时已知能引发中和性和保护性抗体的表位得到了表达，但由任一载体表达的重组包膜蛋白在小鼠或兔子体内均未能诱导出针对跳跃病病毒的中和性或保护性抗体。
