Venugopal K, Jiang W R, Gould E A
Institute of Virology and Environmental Microbiology, Oxford, UK.
Vaccine. 1995 Aug;13(11):1000-5. doi: 10.1016/0264-410x(95)00015-s.
St. Louis encephalitis (SLE) is an important mosquito-borne disease of great public health concern in parts of the United States. South America and Canada. Protective immunogens of flaviviruses produced in different expression systems have been shown to be effective against virulent virus infection in laboratory animal models. Here we show that the pre-membrane and envelope (PrM/E) of SLE virus expressed in insect and mammalian cell systems using baculovirus and vaccinia virus, respectively, are processed correctly and showed similar antigenic characteristics as the authentic proteins. Immunization with the recombinant proteins individually or in combination resulted in neutralizing and protective immune responses. A schedule consisting of initial immunization with recombinant vaccinia virus followed by a secondary boost with recombinant baculovirus protein resulted in higher levels of neutralizing and protective immune responses. The advantages of the use of such a combined approach as a general immunization strategy are discussed.
圣路易斯脑炎（SLE）是一种在美国部分地区、南美洲和加拿大引起重大公共卫生关注的重要蚊媒疾病。在不同表达系统中产生的黄病毒保护性免疫原已被证明在实验室动物模型中对强毒病毒感染有效。在这里，我们表明，分别使用杆状病毒和痘苗病毒在昆虫和哺乳动物细胞系统中表达的SLE病毒的前膜和包膜（PrM/E）被正确加工，并显示出与天然蛋白相似的抗原特性。单独或联合使用重组蛋白进行免疫可产生中和性和保护性免疫反应。由重组痘苗病毒进行初次免疫，随后用重组杆状病毒蛋白进行二次加强免疫的方案可产生更高水平的中和性和保护性免疫反应。讨论了使用这种联合方法作为一般免疫策略的优点。
