Allard A K, Wadell G, Evander K M, Lindman G K
J Virol. 1985 Apr;54(1):145-50. doi: 10.1128/JVI.54.1.145-150.1985.
Enteric adenovirus type 40 (Ad40) and Ad41 form the sixth subgenus of human adenoviruses. They are associated with infantile diarrhea but cannot be isolated in conventional cell cultures. The genome of the fastidious enteric Ad41 has been cloned, and the cleavage sites of the genome produced by restriction endonucleases BamHI, EcoRI, HpaI, NruI, PvuI, and SalI have been mapped. To develop useful hybridization methods for direct detection of adenoviruses, a restriction fragment library containing Ad41 DNA, with plasmid pBR322 as vector, has been constructed. Clones have been isolated which contain 8 of 10 possible BamHI fragments of Ad41, inserted into the BamHI cleavage site of the vector. Two of these clones are particularly useful for the detection of adenoviruses. One clone detects members of all human adenovirus subgenera, and the second clone is specific for enteric adenoviruses, in particular Ad41. A conspicuous absence of detectable homology was noted at 1.5 to 3.3 map units of the Ad41 genome in hybridizations against other serotypes of adenoviruses, including the closely related enteric Ad40. This sequence corresponds to the 5' portion of early region Ia.
肠道腺病毒40型（Ad40）和Ad41型构成了人类腺病毒的第六个亚属。它们与婴儿腹泻有关，但无法在传统细胞培养物中分离出来。挑剔的肠道腺病毒Ad41的基因组已被克隆，并且已经绘制了由限制性内切酶BamHI、EcoRI、HpaI、NruI、PvuI和SalI产生的基因组切割位点图谱。为了开发用于直接检测腺病毒的有用杂交方法，已经构建了一个以质粒pBR322为载体、包含Ad41 DNA的限制性片段文库。已经分离出克隆，这些克隆包含Ad41的10个可能的BamHI片段中的8个，插入到载体的BamHI切割位点中。其中两个克隆对于检测腺病毒特别有用。一个克隆可检测所有人类腺病毒亚属的成员，第二个克隆对肠道腺病毒具有特异性，特别是Ad41。在与其他腺病毒血清型（包括密切相关的肠道腺病毒Ad40）的杂交中，在Ad41基因组的1.5至3.3个图谱单位处明显缺乏可检测到的同源性。该序列对应于早期区域Ia的5'部分。
