Byrd P J, Chia W, Rigby P W, Gallimore P H
J Gen Virol. 1982 Jun;60(Pt 2):279-93. doi: 10.1099/0022-1317-60-2-279.
The human adenovirus serotype 12 (Ad-12) EcoRI-C DNA fragment (0 to 16.5 map units) was cloned in the plasmid vector pAT153. This cloned Ad-12 EcoRI-C DNA fragment was subcloned generating recombinant plasmids which contained the Ad-12 SalI-C fragment (0 to 10.3 map units), the Ad-12 HindIII-G fragment (0 to 6 X 8 map units) and the Ad-12 AccI-H fragment (0 to 4 X 7 map units). Thus, we constructed recombinant plasmids which contain Ad-12 DNA sequences which represent all or part of the virus transforming gene region. The capacity of the cloned Ad-12 EcoRI-C DNA fragment to transform rat cells in vitro was assessed using the focus assay on primary cultures of rat cells. The specific transforming activity of this recombinant plasmid was in the same range as that found for intact Ad-12 DNA. Transformed foci which were induced by the cloned Ad-12 EcoRI-C DNA fragment were established as cell lines and the presence of Ad-12 DNA in these lines was demonstrated using the Southern blotting technique.
人腺病毒12型（Ad-12）的EcoRI-C DNA片段（0至16.5个图谱单位）被克隆到质粒载体pAT153中。这个克隆的Ad-12 EcoRI-C DNA片段被亚克隆，产生了包含Ad-12 SalI-C片段（0至10.3个图谱单位）、Ad-12 HindIII-G片段（0至6×8个图谱单位）和Ad-12 AccI-H片段（0至4×7个图谱单位）的重组质粒。因此，我们构建了包含代表病毒转化基因区域全部或部分的Ad-12 DNA序列的重组质粒。使用大鼠细胞原代培养物上的集落形成试验评估克隆的Ad-12 EcoRI-C DNA片段体外转化大鼠细胞的能力。该重组质粒的特异性转化活性与完整Ad-12 DNA的活性处于同一范围。由克隆的Ad-12 EcoRI-C DNA片段诱导产生的转化集落被建立为细胞系，并使用Southern印迹技术证明这些细胞系中存在Ad-12 DNA。
