Shinozaki T, Araki K, Kobayashi M, Fujita Y, Abe T, Ushijima H
Department of Pediatrics, Teikyo University School of Medicine, Itabashi, Japan.
J Clin Microbiol. 1988 Dec;26(12):2567-71. doi: 10.1128/jcm.26.12.2567-2571.1988.
Nineteen strains of fecal adenovirus were typed as adenovirus type 40 (Ad40) by restriction enzyme analysis of the DNA, using SmaI. The DNAs of the strains were further compared by digestion with four other enzymes, namely, HindIII, EcoRI, BglI, and BamHI. At least two different restriction profiles were found to exist for BamHI. Of the 19 strains studied, 17 had the same combination of profiles for all of the enzymes. Thirteen strains of fecal adenovirus were typed as Ad41 by restriction enzyme analysis of the DNA, using SmaI. The DNAs of the strains were further compared by digestion with four other enzymes, as for Ad40. At least two different restriction profiles were found to exist for each enzyme. Of 13 strains, 9 had the same cleavage sites for all of the enzymes, indicating a close relationship. Two strains were genome variants which deviated from the common genome on digestion of the DNA with HindIII. One strain was a genome variant which deviated from the common genome on digestion of the DNA with HindIII and EcoRI. The last one had a combination of restriction sites which was not identical to those of other strains on digestion of the DNA with SmaI. All 13 Ad41 strains studied were easily distinguished from Ad40 strains by using any of the five restriction enzymes.
通过使用SmaI对DNA进行限制性酶切分析，将19株粪便腺病毒鉴定为腺病毒40型（Ad40）。通过用另外四种酶（即HindIII、EcoRI、BglI和BamHI）消化，进一步比较这些菌株的DNA。发现BamHI至少存在两种不同的限制性图谱。在所研究的19株菌株中，17株对于所有酶具有相同的图谱组合。通过使用SmaI对DNA进行限制性酶切分析，将13株粪便腺病毒鉴定为Ad41。如同对Ad40的研究一样，通过用另外四种酶消化，进一步比较这些菌株的DNA。发现每种酶至少存在两种不同的限制性图谱。在13株菌株中，9株对于所有酶具有相同的切割位点，表明它们关系密切。两株是基因组变体，在用HindIII消化DNA时偏离了共同基因组。一株是在用HindIII和EcoRI消化DNA时偏离共同基因组的基因组变体。最后一株在用SmaI消化DNA时具有与其他菌株不同的限制性位点组合。通过使用这五种限制性酶中的任何一种，所研究的所有13株Ad41菌株都很容易与Ad40菌株区分开来。
