Numazaki K, Nagata N, Sato T, Chiba S
Department of Pediatrics, Sapporo Medical College, Japan.
Med Microbiol Immunol. 1992;181(6):323-31. doi: 10.1007/BF00191544.
Human cytomegalovirus (HCMV) infection in immunocompromized hosts sometimes occurs as a result of reactivation. Cells of the monocyte-macrophage linkage are suggested to be a site of latency and persistence for HCMV. The human monocytic cell line U937 was infected with the AD169 strain and a clinical isolate of HCMV. The expression of surface antigens on the cells was assessed by flow cytometry. The polymerase chain reaction (PCR) was used to detect viral DNA from infected cells. CMV immediate early antigen, early antigen, and late antigen (LA) were detected from both clinical isolate- and AD169-inoculated U937 cells by flow cytometry. CMV DNA which code major immediate early gene (US3) and LA gene (US14) were detected from the clinical isolate-inoculated U937 over a period of 31 days as tested by PCR. These U937 cells proliferated as well as uninfected U937 cell, but only a small number of AD169-inoculated U937 cells survived after 14 days of inoculation. Interleukin-2 activities were detected in the media on days 24-40 after inoculation with AD169. This chronic CMV infection model of U937 might be utilized to study the mechanisms of persistence and reactivation.
免疫功能低下宿主中的人巨细胞病毒（HCMV）感染有时是由于病毒重新激活所致。单核细胞 - 巨噬细胞系的细胞被认为是HCMV潜伏和持续存在的部位。用人单核细胞系U937感染AD169毒株和一株HCMV临床分离株。通过流式细胞术评估细胞表面抗原的表达。采用聚合酶链反应（PCR）检测感染细胞中的病毒DNA。通过流式细胞术在临床分离株和AD169接种的U937细胞中均检测到巨细胞病毒立即早期抗原、早期抗原和晚期抗原（LA）。通过PCR检测发现，在31天的时间里，从临床分离株接种的U937细胞中检测到编码主要立即早期基因（US3）和LA基因（US14）的CMV DNA。这些U937细胞与未感染的U937细胞一样增殖，但接种AD169的U937细胞在接种14天后只有少数存活。接种AD169后第24 - 40天，在培养基中检测到白细胞介素 - 2活性。U937的这种慢性CMV感染模型可用于研究病毒持续存在和重新激活的机制。
