[接种口疮热病毒的小鼠体液免疫反应测量的各种方法比较]

[Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus].

作者信息

Feledi C A, Pujol C A, Arese A, Piccone M E, Marcovecchio F, Zuloaga G, Massouh E J

机构信息

Laboratorio de Inmunoquímica, Facultad de Ciencias Exactas y Naturales, UBA, Argentina.

出版信息

Rev Argent Microbiol. 1992 Jul-Dec;24(3-4):126-35.

PMID:1338879

Abstract

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

摘要

通过酶联免疫吸附测定（ELISA）和被动血凝反应（PH）评估成年CF1小鼠对氢氧化铝-皂苷（AHS）或油乳剂（OE）佐剂的口蹄疫病毒O1坎波斯株的体液免疫初次反应动力学和七天二次反应。对AHS疫苗反应的分析表明，ELISA测得初次反应的最大滴度出现在接种后23天（dpv），二次反应出现在第17天，而PH检测到初次和二次反应的最大滴度出现在接种后约60天。通过ELISA研究的用OE疫苗免疫的小鼠在约40 dpv时出现初次反应平台期，而二次反应在约80 dpv时最大。用PH检测相同血清，初次反应的最高滴度出现在接种后50天，二次反应在第40天最大。基于测定抗体水平所采用的方法，提出了一种在小鼠模型中评估疫苗接种效率的标准。