Kaneko K, Kobayashi H, Onodera O, Miyatake T, Tsuji S
Department of Neurology, Niigata University, Japan.
Hum Mol Genet. 1992 Oct;1(7):529-33. doi: 10.1093/hmg/1.7.529.
Using a cosmid clone derived from human Xq28 as a probe which shows cross-species homology, we isolated cDNA clones and the nucleotide sequence analysis of the cDNA revealed that the cDNA is identical to QM cDNA. The QM cDNA has recently been reported as a cDNA with down-regulation in tumorigenic Wilms' tumor microcell hybrid. Comparison of the nucleotide sequences of the cDNA with those of the genomic DNA allowed us to determine the genomic organization of the QM gene. The QM gene consists of at least 7 exons and is located at Xq28. Southern blot analysis of a somatic cell hybrid panel indicates that the QM genes are scattered at least to chromosome 2, 3, 6, 14, 16, and possibly to other chromosomes. Northern blot analysis demonstrated the QM gene is expressed in all the examined adult human tissues as well as cell lines including HeLa cells, fibroblasts, and somatic cell hybrids with increased expression in liver, spleen, testis, and adrenal gland. The results suggest that the QM gene belongs to a new multi-gene family with yet undetermined function.
我们使用源自人类Xq28的黏粒克隆作为探针，该探针显示出跨物种同源性，分离出了cDNA克隆，并且对该cDNA的核苷酸序列分析表明，此cDNA与QM cDNA相同。QM cDNA最近被报道为在致瘤性肾母细胞瘤微细胞杂种中表达下调的一种cDNA。通过将该cDNA的核苷酸序列与基因组DNA的序列进行比较，我们得以确定QM基因的基因组结构。QM基因至少由7个外显子组成，位于Xq28。对一个体细胞杂种板进行的Southern印迹分析表明，QM基因至少散布到了2号、3号、6号、14号、16号染色体，并且可能还散布到了其他染色体。Northern印迹分析表明，QM基因在所有检测的成人组织以及包括HeLa细胞、成纤维细胞和体细胞杂种在内的细胞系中均有表达，在肝脏、脾脏、睾丸和肾上腺中表达增加。这些结果提示，QM基因属于一个功能尚未确定的新的多基因家族。
