van den Ouweland A M, Verdijk M, Mannens M M, van Oost B A
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
Hum Genet. 1992 Sep-Oct;90(1-2):144-6. doi: 10.1007/BF00210759.
Inactivation of one or more tumor-suppressor genes on the short arm of chromosome 11 is thought to play a role in the etiology of Wilms' tumor. A candidate gene, QM, was recently isolated by subtractive hybridization between a tumorigenic cell line (deleted for part of 11p) and a non-tumorigenic cell line (the tumorigenic cell line carrying an extra t(X;11)copy). We show here with an exon-specific polymerase chain reaction that the genomic homolog of the QM cDNA is located in the G6PD-color vision genes region in Xq28. No homologous sequences could be detected on 11p. Our experiments indicate that the QM gene is not involved in the suppression of Wilms' tumor.
11号染色体短臂上一个或多个肿瘤抑制基因的失活被认为在肾母细胞瘤的病因学中起作用。一个候选基因QM，最近通过一个致瘤细胞系（缺失部分11p）和一个非致瘤细胞系（该致瘤细胞系携带一个额外的t(X;11)拷贝）之间的消减杂交被分离出来。我们在这里用外显子特异性聚合酶链反应表明，QM cDNA的基因组同源物位于Xq28的G6PD-色觉基因区域。在11p上未检测到同源序列。我们的实验表明，QM基因不参与肾母细胞瘤的抑制。
