Dowdy S F, Lai K M, Weissman B E, Matsui Y, Hogan B L, Stanbridge E J
Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine 92717.
Nucleic Acids Res. 1991 Oct 25;19(20):5763-9. doi: 10.1093/nar/19.20.5763.
Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.
肾母细胞瘤是一种儿童肾胚细胞瘤，与11p13和11p15区域的基因改变有关。先前已证明，将一条der(11)染色体导入G401肾母细胞瘤细胞系可使致瘤表型逆转。在致瘤亲本（G401）和含有der(11)染色体的G401非致瘤微细胞杂种（110.1/G401.1）之间进行的消减cDNA/RNA杂交，鉴定出一个单一的新cDNA克隆，命名为QM。该cDNA长度为745个核苷酸，编码一种预测的亲水性25kd碱性蛋白，主要由α螺旋组成。QM转录本在多种胚胎和成年组织中表达，在成年肾脏和心脏中表达下调。QM也是一个多基因家族的成员，该家族成员定位于6号和14号染色体。QM mRNA水平在致瘤和非致瘤细胞系之间受到调节，因此可能参与非致瘤表型的维持。
