Sargeaunt P G
London School of Hygiene & Tropical Medicine, UK.
Arch Med Res. 1992;23(2):265-7.
E. histolytica, pathogenic, and E. dispar, non-pathogenic, being morphologically identical are, when recovered as cysts in feces, diagnosed as E. histolytica. A survey on The Seychelles demonstrated that when culture and zymodeme characterization was used to compare against microscopy alone, the risk of overdiagnosis of E. histolytica infections was drastically reduced. From a total of 313 subjects tested 21 cultures grew amebas. By zymodeme analysis eight were E. histolytica, 40 were E. dispar and the remainder were various species of non-pathogenic intestinal amebas. Two further small comparative surveys confirmed these findings.
致病的溶组织内阿米巴和非致病的迪氏内阿米巴在形态上相同,当在粪便中作为包囊被发现时,都被诊断为溶组织内阿米巴。在塞舌尔进行的一项调查表明,当使用培养和酶谱特征分析并与单纯显微镜检查进行比较时,溶组织内阿米巴感染的过度诊断风险大幅降低。在总共313名接受检测的受试者中,有21份培养物培养出了阿米巴。通过酶谱分析,其中8份为溶组织内阿米巴,40份为迪氏内阿米巴,其余为各种非致病性肠道阿米巴物种。另外两项小型比较调查证实了这些结果。