Seredenko M M, Antonova I I, Koval S B
Fiziol Zh (1978). 1992 Nov-Dec;38(6):20-5.
The experiments on 40 Wistar male rats with acute circulatory-hemic hypoxia induced by hemorrhage in amount of 15-20% of circulating blood volume were carried out to study the state of lysosomal apparatus of the liver and lung tissues as to changes in activity of acid phosphatase (AP), a marker enzyme of lysosomes. In the case of such a hemorrhage the common activity of AP decreased by 37% in the liver tissue and by 41% in the lung tissue, the free activity increased by 30% and 25%, and bound activity decreased by 84 and 70% in homogenates of the corresponding tissues. Under these conditions the activity of the above lysosomal enzyme in blood plasma became five times as higher as control. A conclusion is made concerning the circulatory-hemic hypoxia influence on the lysosomal membrane's structures of organ's tissues cells (as one can judge by the example of liver and lungs), which is observed in an increase of permeability of lysosome membrane and causes a sharp increase of AP enzymia in blood plasma.
对40只雄性Wistar大鼠进行实验，通过放血使循环血量减少15 - 20%来诱导急性循环血液性缺氧，以研究肝脏和肺组织溶酶体装置的状态，观察溶酶体标记酶酸性磷酸酶（AP）活性的变化。在这种出血情况下，肝脏组织中AP的总活性降低了37%，肺组织中降低了41%，相应组织匀浆中游离活性分别增加了30%和25%，结合活性分别降低了84%和70%。在这些条件下，血浆中上述溶酶体酶的活性比对照组高五倍。得出关于循环血液性缺氧对器官组织细胞溶酶体膜结构的影响（以肝脏和肺为例）的结论，即观察到溶酶体膜通透性增加，并导致血浆中AP酶活性急剧升高。
