Kaplan M S, Byers V S, Levin A S, German D F, Fudenberg H H, Lecam L N
J Allergy Clin Immunol. 1976 Jul;58(1 PT. 2):180-9. doi: 10.1016/0091-6749(76)90153-6.
The in vitro measurement of stimulated T lymphocyte blastogenesis by the incorporation of 14C-thymidine can be shown to be an acceptable and precise measurement with highly significant variations in blastogenesis over a 24 hr period. This circadian rhythm for human T lymphocyte blastogenesis has been demonstrated with specific antigen (SKSD) and in mixed leukocyte culture (MLC). The hours of peak reactivity were different for the SKSD stimulation and the MLC reaction. This biologic cycle in T cell reactivity has significant implications for the immunologic response.
通过掺入14C-胸腺嘧啶核苷对刺激的T淋巴细胞增殖进行体外测量，结果表明这是一种可接受且精确的测量方法，在24小时内增殖有高度显著的变化。人类T淋巴细胞增殖的这种昼夜节律已在特异性抗原（SKSD）刺激和混合白细胞培养（MLC）中得到证实。SKSD刺激和MLC反应的反应高峰时间不同。T细胞反应性的这种生物学周期对免疫反应具有重要意义。
