Greene B M, Fanning M M, Ellner J J
Clin Exp Immunol. 1983 May;52(2):259-65.
Lymphocyte blastogenic responses to O. volvulus antigen (Oncho Ag), SKSD, and the mitogen PHA were tested in three groups of persons: light to moderately infected persons (INF); previously exposed but uninfected persons (EXP) and normal controls (NC). The exposed group showed significant responsiveness to Oncho Ag (delta ct/min = 6,002 +/- 1,375), while the infected (delta ct/min = 943 +/- 418) and normal control (delta ct/min = 428 +/- 418) groups did not. The mean blastogenic response to SKSD were EXP, 8,644 +/- 5,249; NC 6,039 +/- 2,880; INF, 2,619 +/- 1,012. The reduced reactivity in the INF group to Oncho Ag showed a significant correlation with reactivity to SKSD (P less than 0.05). To elucidate the mechanism of hyporesponsiveness in the infected group rigorous adherent cell depletion, by adherence to plastic followed by a nylon wool column, was utilized. When 20% plastic adherent cells were added back to the T cells prepared in this fashion, the mean blastogenic response to SKSD was significantly augmented (P less than 0.01). In contrast, the responsiveness to Oncho Ag was not significantly altered. The addition of indomethacin (1 microgram/ml) or autologous plasma had no significant effect on reactivity to either SKSD or Oncho Ag. There were no significant differences in the mean reactivity of the three groups to PHA-M (delta ct/min EXP 78,514 +/- 12,564; INF 62,393 +/- 14,447; NC 61,423 +/- 4,465). These results suggest that O. volvulus infection is associated with decreased lymphocyte reactivity to both parasite related and unrelated antigens, and imply that the mechanism for the two types of hyporesponsiveness may be distinct. While a weakly adherent suppressor cell may account for non-specific hyporesponsiveness, the mechanism of parasite specific decreased reactivity remains unknown.
在三组人群中检测了淋巴细胞对旋盘尾丝虫抗原（盘尾丝虫抗原）、SKSD和丝裂原PHA的增殖反应：轻度至中度感染人群（INF）；既往暴露但未感染人群（EXP）和正常对照组（NC）。暴露组对盘尾丝虫抗原有显著反应性（δct/分钟 = 6,002 ± 1,375），而感染组（δct/分钟 = 943 ± 418）和正常对照组（δct/分钟 = 428 ± 418）则无反应。对SKSD的平均增殖反应分别为：EXP组8,644 ± 5,249；NC组6,039 ± 2,880；INF组2,619 ± 1,012。INF组对盘尾丝虫抗原反应性降低与对SKSD的反应性呈显著相关性（P < 0.05）。为阐明感染组低反应性的机制，采用了通过贴壁于塑料培养皿随后经尼龙毛柱处理进行严格的黏附细胞清除方法。当将20%的塑料贴壁细胞重新添加到以此方式制备的T细胞中时，对SKSD的平均增殖反应显著增强（P < 0.01）。相比之下，对盘尾丝虫抗原的反应性无显著改变。添加吲哚美辛（1微克/毫升）或自体血浆对SKSD或盘尾丝虫抗原的反应性均无显著影响。三组对PHA-M的平均反应性无显著差异（δct/分钟：EXP组78,514 ± 12,564；INF组62,393 ± 14,447；NC组61,423 ± 4,465）。这些结果表明，盘尾丝虫感染与淋巴细胞对寄生虫相关和不相关抗原的反应性降低有关，并且意味着这两种低反应性的机制可能不同。虽然弱黏附抑制细胞可能导致非特异性低反应性，但寄生虫特异性反应性降低的机制仍然未知。
