Ruckdeschel J C, Doukas J G, Drake W P, Mardiney M R
Transplantation. 1977 May;23(5):396-403. doi: 10.1097/00007890-197705000-00003.
This study defines an assay (laser analysis) that is a significant advance in our ability to quantitate and analyze immunologically induced in vitro lymphocyte responsiveness. Laser analysis is demonstrated to parallel radionucleotide incorporation (3H-thymidine) in terms of kinetic pattern, dose response characteristics, and statistical accuracy while exeeding radionucleotide incorporation in sensitivity. Direct quantitation of lymphocyte responsiveness, in terms of cellular proliferation, disclosed that substantial numbers of small lymphocytes were produced during in vitro stimulation with mitogen (concanavalin A) or antigen (streptokinase-streptodornase) in addition to the expected increase in lymphoblasts. The magnitude of this "total cellular response" (lymphocytes plus lymphoblasts) was found to be similar for antigen and mitogen stimulation, a finding not suggested by routine radionucleotide incorporation or morphological assays.
本研究定义了一种检测方法（激光分析），这在我们定量和分析免疫诱导的体外淋巴细胞反应性的能力方面是一项重大进展。结果表明，激光分析在动力学模式、剂量反应特征和统计准确性方面与放射性核苷酸掺入（³H-胸腺嘧啶核苷）相似，而在灵敏度方面超过了放射性核苷酸掺入。就细胞增殖而言，对淋巴细胞反应性的直接定量显示，在用有丝分裂原（刀豆球蛋白A）或抗原（链激酶-链道酶）进行体外刺激期间，除了预期的成淋巴细胞增加外，还产生了大量小淋巴细胞。发现这种“总细胞反应”（淋巴细胞加成淋巴细胞）对抗原和有丝分裂原刺激的程度相似，这一发现是常规放射性核苷酸掺入或形态学检测未表明的。
