Brahm J, Hurtado C, Latorre R, Velasco M
Centro de Gastroenterología, Hospital Clínico Universidad de Chile.
Rev Med Chil. 1992 Sep;120(9):1003-5.
The DNA-HBV was investigated in 109 serum samples from 75 patients with different forms of infection with the HB virus, using an RIA hybridization technique (Genostic-Abott). DNA-HBV was present in 12 of 18 cases of acute hepatitis, 1 of 3 patients with fulminant disease, 11 of 14 patients with chronic hepatitis and/or cirrhosis, 2 of 10 patients with hepatoma and 6 of 30 asymptomatic chronic carriers. Presence of DNA-HBV beyond 60 days in 7 patients with acute hepatitis established the chronic state. The highest levels were found in patients with chronic hepatitis or cirrhosis (mean 41 +/- 11 pg/ml) and the lowest in chronic carriers and patients with hepatoma. High levels of DNA-HBV denote persistent viral replication and would support antiviral treatment. Thus, investigation of DNA-HBV has diagnostic, prognostic and therapeutic implications in patients with Hepatitis B.
采用放射免疫分析杂交技术（Genostic - Abbott），对75例感染乙肝病毒不同形式的患者的109份血清样本进行了乙肝病毒DNA（DNA - HBV）检测。在18例急性肝炎患者中有12例检测到DNA - HBV，3例暴发性疾病患者中有1例检测到，14例慢性肝炎和/或肝硬化患者中有11例检测到，10例肝癌患者中有2例检测到，30例无症状慢性携带者中有6例检测到。7例急性肝炎患者在60天之后仍检测到DNA - HBV，这表明已转为慢性状态。DNA - HBV水平最高的是慢性肝炎或肝硬化患者（平均41±11 pg/ml），最低的是慢性携带者和肝癌患者。DNA - HBV高水平表明病毒持续复制，支持进行抗病毒治疗。因此，对DNA - HBV的检测对乙肝患者具有诊断、预后和治疗意义。
