Kokkinopoulos D, Perez S, Papamichail M
Blut. 1985 Jun;50(6):341-8. doi: 10.1007/BF00320927.
Thymosin beta 4 was tested for its ability to induce phenotypic changes in the human T-cell line Molt-4. Cells were cultured with nanogram concentrations of thymosin beta 4 for up to 16 days and were analyzed with a panel of monoclonal antibodies, sheep erythrocyte rosetting, peanut agglutinin binding (PNA) and an antibody to the enzyme, terminal deoxynucleotidyl transferase (TdT). Thymosin beta 4 induced Molt-4 cells to reduce the expression of a T-cell lineage specific antigen, with preferential expression on T blast-cells, detected by WT 1 monoclonal antibody. Thymosin beta 4 also induced an increase in sheep erythrocyte rosettes and PNA binding as well as an increased expression of OKT 11 A and OKT 8 in Molt-4 cells. TdT was found to be unchanged, however. Analysis of thymosin beta 4-treated cells with other monoclonal antibodies (OKT 3, OKT 6, OKT 9) showed no change when compared to controls. These results showed that thymosin beta 4 is capable of inducing phenotypic changes in Molt-4 cells. Such changes may represent a differentiation process of these cells through the early stages of the maturation process of thymus-dependent lymphocytes, albeit not to the stage of mature T cells.
检测了胸腺素β4诱导人T细胞系Molt-4表型变化的能力。将细胞与纳克浓度的胸腺素β4一起培养长达16天，并用一组单克隆抗体、绵羊红细胞玫瑰花结试验、花生凝集素结合试验（PNA）以及针对末端脱氧核苷酸转移酶（TdT）的抗体进行分析。胸腺素β4诱导Molt-4细胞降低一种T细胞系特异性抗原的表达，该抗原在T母细胞上优先表达，可通过WT 1单克隆抗体检测到。胸腺素β4还诱导Molt-4细胞中绵羊红细胞玫瑰花结和PNA结合增加，以及OKT 11 A和OKT 8的表达增加。然而，发现TdT没有变化。用其他单克隆抗体（OKT 3、OKT 6、OKT 9）分析胸腺素β4处理的细胞，与对照相比没有变化。这些结果表明胸腺素β4能够诱导Molt-4细胞发生表型变化。这种变化可能代表这些细胞通过胸腺依赖性淋巴细胞成熟过程的早期阶段发生分化，尽管尚未达到成熟T细胞阶段。
