Hagmar B, Johansson B, Kalantari M, Petersson Z, Skyldberg B, Walaas L
Department of Pathology, University of Göteborg, Laboratory of Clinical Microbiology, Stockholm, Sweden.
Med Oncol Tumor Pharmacother. 1992;9(3):113-7. doi: 10.1007/BF02987743.
The incidence of HPV was studied in 71 invasive squamous carcinomas of the cervix using PCR technique. We used primers, which presumably recognize all types of HPV (consensus primers), and also type-specific primers. In situ hybridization was carried out in 24 of the cases. The overall incidence of HPV was 53/71 (75%) of which 5 cases were positive with the consensus primers only. However, 21/71 cases (30%) were negative for the consensus primers but positive for one of the type-specific primer pairs. This finding indicates that subgenomic deletions may have occurred in the viral genome upon integration in the human DNA. In situ hybridization was positive in 14/24 cases (58%), showing excellent correlation with PCR results. The HPV types detected were, in descending order of frequency: type 16 (52%), 31 (23%), 18 (13%), 33 (12%). No cases of HPV type 6 or 11 were found in this series of invasive carcinomas.
采用聚合酶链反应（PCR）技术对71例宫颈浸润性鳞状细胞癌患者的人乳头瘤病毒（HPV）感染情况进行了研究。我们使用了可能识别所有HPV类型的引物（通用引物）以及型特异性引物。对其中24例病例进行了原位杂交。HPV的总体感染率为53/71（75%），其中5例仅通用引物检测呈阳性。然而，21/71例（30%）通用引物检测为阴性，但某一型特异性引物对检测呈阳性。这一发现表明，病毒基因组在整合入人类DNA时可能发生了亚基因组缺失。原位杂交在14/24例（58%）中呈阳性，与PCR结果显示出良好的相关性。检测到的HPV类型按频率从高到低依次为：16型（52%）、31型（23%）、18型（13%）、33型（12%）。在这一系列浸润性癌中未发现6型或11型HPV感染病例。
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