新加坡子宫颈发育异常和癌中的人乳头瘤病毒

Human papillomavirus in dysplasia and carcinoma of the cervix in Singapore.

作者信息

Lim-Tan S K, Yoshikawa H, Sng I T, de Villiers E M, zur Hausen H, Ho T H, Yoong T

机构信息

Referenzzentrum für humanpathogene Papillomviren/ATV im Deutschen Krebsforschungszentrum, Federal Republic of Germany.

出版信息

Pathology. 1988 Oct;20(4):317-9. doi: 10.3109/00313028809085210.

DOI:10.3109/00313028809085210

PMID:2853859

Abstract

A prospective study was conducted in Singapore in 1985 where 107 women with abnormal cervical smears were studied for cervical neoplasia and its association with the human papillomaviruses (HPV), using HPV 11, 16 and 18 DNA as probes. Cervical biopsies were performed for histology as well as for DNA Southern Blot hybridization studies to detect the presence of HPV 11, 16 or 18 genome. The prevalence of the various types of papillomavirus DNA in cervical tissue samples from cervical carcinoma and dysplasias is presented. HPV types 16 and 18 were found in 5 of 8 (63%) cases of invasive squamous cell carcinoma (SCC), in 12 of 41 (29%) cases of squamous carcinoma-in-situ (CIN 3) and in 3 of 48 (6%) cases of lower grade dysplasia. None of our cases of SCC were associated with unknown HPV types detected using a mixture of HPV 16 or 18 DNA under conditions of low stringency. These unknown HPV types were present in approximately one quarter of our cases of dysplasia of all grades.

摘要

1985年在新加坡进行了一项前瞻性研究，研究对象为107名宫颈涂片异常的女性，以研究宫颈癌前病变及其与人乳头瘤病毒（HPV）的关联，使用HPV 11、16和18 DNA作为探针。对宫颈活检组织进行组织学检查以及DNA Southern印迹杂交研究，以检测HPV 11、16或18基因组的存在。本文呈现了宫颈癌和发育异常的宫颈组织样本中各种类型乳头瘤病毒DNA的患病率。在8例浸润性鳞状细胞癌（SCC）中有5例（63%）、41例原位鳞状癌（CIN 3）中有12例（29%）以及48例低级别发育异常中有3例（6%）检测到HPV 16和18型。在低严谨条件下，使用HPV 16或18 DNA混合物检测时，我们所有的SCC病例均未与未知HPV类型相关。这些未知HPV类型存在于我们所有级别的发育异常病例的约四分之一中。