Haakenstad A O, Mannik M
Lab Invest. 1976 Sep;35(3):283-92.
Soluble immune complexes prepared with reduced and alkylated antibodies persisted longer in the circulation than complexes prepared with intact antibodies, when these were administered intravenously to mice. The disappearance of complexes with reduced and alkylated antibodies was delayed in part because the initial phase of vascular permeability was considerably less than that seen following the administration of complexes with intact antibodies. In addition, large complexes with lattice structure of more than two antigen and two antibody molecules persisted longer in the circulation after administration of complexes with reduced and alkylated antibodies than after administration of complexes with intact antibodies. Thus, the concentration of large latticed complexes with reduced and alkylated antibodies was significantly greater than the concentrations of large latticed complexes with intact antobodies at all observed times through 96 hours. The persistence of large latticed complexes with reduced and alkylated antibodies was associated with significantly decreased hepatic localization of complexes with reduced and alkylated antibodies compared to the hepatic localization of complexes with intact antibodies at 1, 4, 12, and 24 hours. The observations indicated that the removal of large latticed complexes from the circulation by the hepatic mononuclear phagocyte system was decreased when reduced and alkylated antibodies were used for the preparation of immune complexes. The persistence of large latticed complexes with reduced and alkylated antibodies in the circulation was associated with enhanced and prolonged presence of glomerular deposits of immune complexes, as reported in the accompanying article (Haakenstad AO, Striker GE, Mannik M: Lab Invest 35:293, 1976.
当将用还原和烷基化抗体制备的可溶性免疫复合物静脉注射给小鼠时，其在循环中的持续时间比用完整抗体制备的复合物更长。用还原和烷基化抗体形成的复合物的消失延迟，部分原因是血管通透性的初始阶段明显低于注射完整抗体形成的复合物后所见的情况。此外，具有超过两个抗原和两个抗体分子晶格结构的大复合物，在用还原和烷基化抗体形成的复合物注射后在循环中的持续时间比用完整抗体形成的复合物注射后更长。因此，在96小时内的所有观察时间，用还原和烷基化抗体形成的大晶格复合物的浓度显著高于用完整抗体形成的大晶格复合物的浓度。与完整抗体形成的复合物在1、4、12和24小时的肝脏定位相比，用还原和烷基化抗体形成的大晶格复合物的持续存在与用还原和烷基化抗体形成的复合物的肝脏定位显著降低有关。这些观察结果表明，当用还原和烷基化抗体制备免疫复合物时，肝脏单核吞噬细胞系统从循环中清除大晶格复合物的能力下降。如随附文章（Haakenstad AO，Striker GE，Mannik M：Lab Invest 35：293，1976）所报道，用还原和烷基化抗体形成的大晶格复合物在循环中的持续存在与免疫复合物肾小球沉积物的增加和持续存在有关。
