Chang K L, Chen Y Y, Shibata D, Weiss L M
Department of Pathology, City of Hope National Medical Center, Duarte, California.
Diagn Mol Pathol. 1992 Dec;1(4):246-55.
The authors describe a highly sensitive and practical in situ hybridization method using an oligonucleotide probe for EBER1 RNA for the detection of Epstein-Barr virus (EBV) in formalin-fixed, paraffin-embedded tissue sections. Paraffin-embedded tissues from 793 cases of normal and neoplastic tissues were studied. Nuclear staining for EBV RNA was uniformly present in all or virtually all neoplastic cells in a variety of known EBV-positive tumors. We also demonstrate rare EBV-infected cells in normal lymphoid tissues. RNAase predigestion, competitive inhibition, and control probe studies confirmed the specificity of the staining. In addition, cross-reactivity of EBV RNA staining with other viruses was not present. Additionally, the distribution of EBV in a wide variety of other normal and neoplastic tissues is reported.
作者描述了一种高度灵敏且实用的原位杂交方法，该方法使用针对EBER1 RNA的寡核苷酸探针，用于在福尔马林固定、石蜡包埋的组织切片中检测爱泼斯坦-巴尔病毒（EBV）。对793例正常和肿瘤组织的石蜡包埋组织进行了研究。在各种已知的EBV阳性肿瘤中，所有或几乎所有肿瘤细胞中均一致出现EBV RNA的核染色。我们还在正常淋巴组织中发现了罕见的EBV感染细胞。核糖核酸酶预消化、竞争性抑制和对照探针研究证实了染色的特异性。此外，EBV RNA染色与其他病毒不存在交叉反应。此外，还报告了EBV在多种其他正常和肿瘤组织中的分布情况。
