Nuovo M A, Nuovo G J, MacConnell P, Forde A, Steiner G C
Department of Pathology, SUNY at Stony Brook 11794.
Diagn Mol Pathol. 1992 Dec;1(4):256-65.
Paget's disease of bone is a disease of unknown etiology. The demonstration of viral-like particles on ultrastructural examination and the putative detection of viral antibodies and nucleic acids in the tissues suggest a possible viral association. The purpose of this study was to search for nucleic acid sequences homologous to measles virus using the recently described reverse transcriptase (RT) polymerase chain reaction (PCR) in situ hybridization (ISH) technique. After performing RT PCR ISH utilizing primers specific for the nucleocapsid region of the measles virus, an intense signal was evident in most measles-infected HeLa cells compared with a weak signal in few of these cells using standard cDNA-RNA ISH analysis. Amplified measles nucleic acid was detected in tissue from a patient who died of measles infection and was not detected in any of the 11 cases of Paget's disease of bone studied or in a giant cell tumor of bone that had tubuloreticular inclusions on electron microscopy. Therefore, these data suggest that infection by the measles virus is not associated with Paget's disease of bone.
骨佩吉特病是一种病因不明的疾病。超微结构检查中发现病毒样颗粒，以及在组织中推测检测到病毒抗体和核酸，提示可能与病毒有关。本研究的目的是使用最近描述的逆转录酶（RT）聚合酶链反应（PCR）原位杂交（ISH）技术，寻找与麻疹病毒同源的核酸序列。在使用针对麻疹病毒核衣壳区域的特异性引物进行RT PCR ISH后，与使用标准cDNA-RNA ISH分析时少数细胞中的微弱信号相比，大多数感染麻疹的HeLa细胞中出现了强烈信号。在死于麻疹感染的患者组织中检测到扩增的麻疹核酸，而在研究的11例骨佩吉特病病例或电子显微镜下有管状网状包涵体的骨巨细胞瘤中均未检测到。因此，这些数据表明麻疹病毒感染与骨佩吉特病无关。
