Birch M A, Taylor W, Fraser W D, Ralston S H, Hart C A, Gallagher J A
Department of Human Anatomy and Cell Biology, The University, Liverpool, England.
J Bone Miner Res. 1994 Jan;9(1):11-6. doi: 10.1002/jbmr.5650090103.
It has been proposed that Paget's disease of bone is caused by the infection of bone cells with one or several paramyxoviruses. In this study we used the polymerase chain reaction (PCR), which allows the detection of very low levels of a target nucleic acid sequence, to study cultures of pagetic bone cells and samples of pagetic bone. Oligonucleotide primers were designed to flank a sequence of the nucleocapsid genome of measles virus and canine distemper virus (CDV). Within this fragment there were contrasting restriction endonuclease sites specific to measles or CDV that allowed identification of the original template. We were unable to detect paramyxovirus RNA in four strains of human bone cells outgrown from pagetic bone and one strain derived from an uninvolved site of a patient with Paget's disease. Furthermore, paramyxovirus sequences were not detected in cDNA prepared from six samples of pagetic bone biopsies. The work presented here further questions the role of measles and CDV in the abnormal remodeling observed in Paget's disease.
有人提出，骨Paget病是由一种或几种副粘病毒感染骨细胞引起的。在本研究中，我们使用聚合酶链反应（PCR）来研究Paget骨细胞培养物和Paget骨样本，该技术能够检测极低水平的目标核酸序列。设计了寡核苷酸引物，使其位于麻疹病毒和犬瘟热病毒（CDV）核衣壳基因组序列的两侧。在该片段内，有麻疹病毒或CDV特有的限制性内切酶位点，可用于鉴定原始模板。我们无法在从Paget骨生长出的四株人骨细胞系以及来自一名Paget病患者未受累部位的一株细胞系中检测到副粘病毒RNA。此外，在从六份Paget骨活检样本制备的cDNA中未检测到副粘病毒序列。本文所呈现的研究结果进一步质疑了麻疹病毒和CDV在Paget病中观察到的异常重塑中的作用。
