Borges M M, De Andrade S G, Pilatti C G, do Prado Júnior J C, Kloetzel J K
Divisão de Biologia Médica, Instituto Adolfo Lutz, São Paulo, Brasil.
Mem Inst Oswaldo Cruz. 1992 Oct-Dec;87(4):493-502. doi: 10.1590/s0074-02761992000400006.
Peritoneal macrophage activation as measured by H2O2 release and histopathology was compared between Swiss mice and Calomys callosus, a wild rodent, reservoir of Trypanosoma cruzi, during the course of infection with four strains of this parasite. In mice F and Y strain infections result in high parasitemia and mortality while with silvatic strains Costalimai and M226 parasitemia is sub-patent, with very low mortality. H2O2 release peaked at 33.6 and 59 nM/2 x 10(6) cells for strains Y and F, respectively, 48 and 50 nM/2 x 10(6) for strains Costalimai and M226, at different days after infection. Histopathological findings of myositis, myocarditis, necrotizing arteritis and absence of macrophage parasitism were found for strains F and Costalimai. Y strain infection presented moderate myocarditis and myositis, with parasites multiplying within macrophages. In C. callosus all four strains resulted in patent parasitemia which was eventually overcome, with scarce mortality. H2O2 release for strains Y and F was comparable to that of mice-peaks of 27 and 53 nM/2 x 10(6) cells, with lower values for strains Costalimai and M226-16.5 and 4.6 nM/2 x 10(6) cells, respectively. Histopathological lesions with Y and F strain injected animals were comparable to those of mice at the onset of infections; they subsided completely at the later stages with Y strain and partially with F strain infected C. callosus. In Costalimai infected C. callosus practically no histopathological alterations were observed.
在感染四种克氏锥虫菌株的过程中，比较了瑞士小鼠和野生啮齿动物卡洛米斯棉鼠（克氏锥虫的储存宿主）之间通过过氧化氢（H₂O₂）释放量和组织病理学测量的腹膜巨噬细胞活化情况。在小鼠中，F株和Y株感染导致高寄生虫血症和死亡率，而对于森林菌株科斯塔利迈株和M226株，寄生虫血症为亚显性，死亡率极低。感染后不同天数，Y株和F株的H₂O₂释放量分别在33.6和59 nM/2×10⁶个细胞时达到峰值，科斯塔利迈株和M226株分别为48和50 nM/2×10⁶个细胞。F株和科斯塔利迈株出现了肌炎、心肌炎、坏死性动脉炎的组织病理学发现，且巨噬细胞内无寄生虫寄生。Y株感染表现为中度心肌炎和肌炎，寄生虫在巨噬细胞内繁殖。在卡洛米斯棉鼠中，所有四种菌株均导致显性寄生虫血症，但最终被克服，死亡率很低。Y株和F株的H₂O₂释放量与小鼠相当——峰值分别为27和53 nM/2×10⁶个细胞，科斯塔利迈株和M226株的值较低，分别为16.5和4.6 nM/2×10⁶个细胞。注射Y株和F株的动物的组织病理学病变在感染开始时与小鼠相似；在后期，Y株感染的卡洛米斯棉鼠病变完全消退，F株感染的部分消退。在感染科斯塔利迈株的卡洛米斯棉鼠中，几乎未观察到组织病理学改变。
