Pereira-Chioccola V L, Schenkman S, Kloetzel J K
Laboratório de Xenodiagnóstico, Instituto Dante Pazzanese de Cardiologia, São Paulo, Brazil.
Infect Immun. 1994 Jul;62(7):2973-8. doi: 10.1128/iai.62.7.2973-2978.1994.
We investigated whether sera from chronic Chagasic patients and animals infected with Trypanosoma cruzi inhibit the removal of sialic acid from human erythrocytes and the transfer of sialic acid from sialyllactose to [14C]lactose in the reactions catalyzed by the parasite trans-sialidase. Sera from Swiss mice and Calomys callosus animals infected with three different T. cruzi strains inhibit both reactions. Inhibition increases during the infection, reaching maximal levels when the parasitemia decreases. Among 44 sera of untreated chronic Chagasic patients, 40 inhibit both reactions. Inhibition is observed with total, defatted sera or with purified immunoglobulins. Whereas most of the inhibitory antibodies from Chagasic patients react with the papain fragment of trans-sialidase in immunoblots, a few patients have noninhibitory antibodies that react only with the entire trans-sialidase. These findings may be relevant for the pathology of Chagas' disease.
我们研究了慢性恰加斯病患者以及感染克氏锥虫的动物的血清，在寄生虫转唾液酸酶催化的反应中，是否会抑制人红细胞上唾液酸的去除以及唾液酸从唾液乳糖向[14C]乳糖的转移。感染三种不同克氏锥虫菌株的瑞士小鼠和加氏鹿鼠的血清会抑制这两种反应。在感染过程中抑制作用增强，当寄生虫血症降低时达到最高水平。在44例未经治疗的慢性恰加斯病患者的血清中，40例抑制这两种反应。在全血清、脱脂血清或纯化的免疫球蛋白中均观察到抑制作用。虽然恰加斯病患者的大多数抑制性抗体在免疫印迹中与转唾液酸酶的木瓜蛋白酶片段发生反应，但少数患者有仅与完整转唾液酸酶发生反应的非抑制性抗体。这些发现可能与恰加斯病病理学相关。
