Ochoa-Sánchez Alicia, Jiménez Lucía, Landa Abraham
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2do piso, Ciudad Universitaria, 04510 México, DF, Mexico.
J Biomed Biotechnol. 2011;2011:504959. doi: 10.1155/2011/504959. Epub 2011 Dec 29.
Humans acquire taeniasis by ingesting pork meat infected with Taenia solium cysticerci, which are the only definitive hosts of the adult stage (tapeworm) and responsible for transmitting the human and porcine cysticercosis. Hence, detection of human tapeworm carriers is a key element in the development of viable strategies to control the disease. This paper presents the identification of specific antigens using sera from hamsters infected with T. solium tapeworms analyzed by western blot assay with crude extracts (CEs) and excretion-secretion antigens (E/S Ag) obtained from T. solium cysticerci and tapeworms and extracts from other helminthes as controls. The hamster sera infected with T. solium tapeworms recognized specific bands of 72, 48, 36, and 24 kDa, in percentages of 81, 81, 90, and 88%, respectively, using the T. solium tapeworms E/S Ag. The antigens recognized by these hamster sera could be candidates to improve diagnosis of human T. solium taeniasis.
人类通过摄入感染有猪带绦虫囊尾蚴的猪肉而感染猪带绦虫病，猪是成虫阶段（绦虫）的唯一终末宿主，并会传播人和猪的囊尾蚴病。因此，检测人体绦虫携带者是制定可行的疾病控制策略的关键因素。本文介绍了使用感染猪带绦虫的仓鼠血清，通过蛋白质印迹分析来鉴定特异性抗原，该分析采用了从猪带绦虫囊尾蚴和绦虫获得的粗提物（CEs）和排泄分泌抗原（E/S Ag），并以其他蠕虫的提取物作为对照。使用猪带绦虫E/S Ag时，感染猪带绦虫的仓鼠血清分别以81%、81%、90%和88%的比例识别出72、48、36和24 kDa的特异性条带。这些仓鼠血清识别的抗原可能是改善人体猪带绦虫病诊断的候选抗原。
