Tanigami A, Tokino T, Takiguchi S, Mori M, Glaser T, Park J W, Jones C, Nakamura Y
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
Am J Hum Genet. 1992 Jan;50(1):56-64.
We have extended our mapping effort on human chromosome 11 to encompass a total of 262 DNA markers, which have been mapped into 24 intervals on chromosome 11; 123 of the markers reveal RFLPs. These clones are scattered throughout the chromosome, although some clustering occurs in R-positive bands (p15.1, p11.2, q13, and q23.3). Fifty-two of the markers were found to contain DNA sequences conserved in Chinese hamster, and some of these 52 also cross-hybridized with DNA from other mammals and/or chicken. As the length of chromosome 11 is estimated at nearly 130 cM, the average distance between RFLP markers is roughly 1 cM. The large panel of DNA markers on our map should contribute to investigations of hereditary diseases on this chromosome, and it will also provide reagents for constructing either fine-scale linkage and physical maps or contig maps of cosmids or yeast artificial chromosomes.
我们已将人类11号染色体的图谱绘制工作扩展到总共262个DNA标记，这些标记已被定位到11号染色体的24个区间；其中123个标记显示出限制性片段长度多态性（RFLP）。这些克隆分散在整个染色体上，不过在R阳性带（p15.1、p11.2、q13和q23.3）存在一些聚集现象。发现其中52个标记含有在中国仓鼠中保守的DNA序列，这52个标记中的一些还与其他哺乳动物和/或鸡的DNA发生交叉杂交。由于估计11号染色体的长度接近130厘摩（cM），RFLP标记之间的平均距离约为1 cM。我们图谱上的大量DNA标记应有助于对该染色体上的遗传性疾病进行研究，还将为构建精细尺度的连锁图谱和物理图谱或黏粒或酵母人工染色体的重叠群图谱提供试剂。
