Sugasawa K, Ishimi Y, Eki T, Hurwitz J, Kikuchi A, Hanaoka F
Cellular Physiology Laboratory, RIKEN Institute, Saitama, Japan.
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1055-9. doi: 10.1073/pnas.89.3.1055.
Simian virus 40 chromosomes can be replicated in vitro with the same set of purified proteins required for the replication of naked DNA containing the viral origin. With these reconstituted systems, the fate of parental histones during replication was examined in vitro. The assembly of nucleosomes on replicating chromosomes was hardly affected by the presence of simultaneously replicating naked DNA competitor, suggesting that replication forks can traverse nucleosomes without the displacement of histones. Moreover, we demonstrate that the nascent nucleosomes were distributed almost equally between the leading and lagging strands. This distributive mode of nucleosome segregation favors the propagation of parental chromatin structures to both daughter cells, which can maintain cellular functions dictated by these structures during cell proliferation.
猴病毒40染色体可在体外利用含有病毒起源的裸露DNA复制所需的同一组纯化蛋白进行复制。利用这些重构系统，在体外研究了复制过程中亲代组蛋白的命运。同时复制的裸露DNA竞争者的存在几乎不影响复制染色体上核小体的组装，这表明复制叉可以穿过核小体而不使组蛋白移位。此外，我们证明新生核小体在前导链和后随链之间几乎平均分配。这种核小体分离的分配模式有利于亲代染色质结构向两个子细胞的传播，这可以在细胞增殖过程中维持由这些结构决定的细胞功能。
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