DNA 上的分子拥堵。
Molecular traffic jams on DNA.
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
出版信息
Annu Rev Biophys. 2013;42:241-63. doi: 10.1146/annurev-biophys-083012-130304. Epub 2013 Feb 28.
Abstract
All aspects of DNA metabolism-including transcription, replication, and repair-involve motor enzymes that move along genomic DNA. These processes must all take place on chromosomes that are occupied by a large number of other proteins. However, very little is known regarding how nucleic acid motor proteins move along the crowded DNA substrates that are likely to exist in physiological settings. This review summarizes recent progress in understanding how DNA-binding motor proteins respond to the presence of other proteins that lie in their paths. We highlight recent single-molecule biophysical experiments aimed at addressing this question, with an emphasis placed on analyzing the single-molecule, ensemble biochemical, and in vivo data from a mechanistic perspective.
摘要
DNA 代谢的各个方面——包括转录、复制和修复——都涉及沿着基因组 DNA 移动的马达酶。这些过程都必须在染色体上进行，而染色体上还占据着大量其他的蛋白质。然而，对于核酸马达蛋白如何沿着可能存在于生理环境中的拥挤 DNA 底物移动，我们知之甚少。这篇综述总结了在理解 DNA 结合马达蛋白如何应对其行进路径中存在的其他蛋白质方面的最新进展。我们强调了最近旨在解决这个问题的单分子生物物理实验，重点从机械角度分析单分子、整体生化和体内数据。
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