利用聚合酶链反应检测脱落宫颈细胞中的16型和18型人乳头瘤病毒

Detection of human papillomavirus types 16 and 18 in the exfoliated cervical cells using the polymerase chain reaction.

作者信息

Nakazawa A, Inoue M, Saito J, Sasagawa T, Ueda G, Tanizawa O

机构信息

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

出版信息

Int J Gynaecol Obstet. 1992 Jan;37(1):13-8. doi: 10.1016/0020-7292(92)90972-l.

DOI:10.1016/0020-7292(92)90972-l

PMID:1346595

Abstract

We applied the polymerase chain reaction (PCR) to detect HPV 16 and 18 in cytological samples obtained from the uterine cervices of Japanese women. HPV infection was detected in 17 (25%) of 67 with CIN and 11 (37%) of 30 with cervical carcinoma. It is notable that 11 (16%) of 69 women with normal cervices were infected with either HPV 16 or 18. The polymerase chain reaction is sensitive and useful for epidemiological studies.

摘要

我们应用聚合酶链反应（PCR）检测从日本女性子宫颈获取的细胞学样本中的人乳头瘤病毒16型（HPV 16）和18型（HPV 18）。在67例宫颈上皮内瘤变（CIN）患者中，有17例（25%）检测到HPV感染；在30例宫颈癌患者中，有11例（37%）检测到HPV感染。值得注意的是，69例宫颈正常的女性中有11例（16%）感染了HPV 16或HPV 18。聚合酶链反应对于流行病学研究来说灵敏且实用。