克隆DNA片段特定突变的快速筛选。 - Suppr

Rapid screening of cloned DNA fragments for specific mutations.

作者信息

Theroux S J, Davis R J

机构信息

Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester 01605.

出版信息

Nucleic Acids Res. 1992 Feb 25;20(4):915. doi: 10.1093/nar/20.4.915.

DOI:10.1093/nar/20.4.915

PMID:1347419

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC312042/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a7a/312042/71dbd688e7a2/nar00078-0260-a.jpg

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Rapid screening of cloned DNA fragments for specific mutations.克隆DNA片段特定突变的快速筛选。

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本文引用的文献

Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.寡核苷酸定向诱变：一种使用两条寡核苷酸引物和单链DNA模板的简单方法。

DNA. 1984 Dec;3(6):479-88. doi: 10.1089/dna.1.1984.3.479.

Site-directed mutagenesis by overlap extension using the polymerase chain reaction.利用聚合酶链反应通过重叠延伸进行定点诱变。

Gene. 1989 Apr 15;77(1):51-9. doi: 10.1016/0378-1119(89)90358-2.

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Rapid screening of cloned DNA fragments for specific mutations.

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