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人类胸腺细胞对CD2抗体的不同反应：CD45RO+亚群的DNA片段化及CD45RO-亚群的增殖。

Differential response of human thymus cells to CD2 antibodies: fragmentation of DNA of CD45RO+ and proliferation of CD45RO- subsets.

作者信息

Li J, Campbell D, Hayward A R

机构信息

Department of Pediatrics, University of Colorado School of Medicine, Denver.

出版信息

Immunology. 1992 Feb;75(2):305-10.

PMID:1348052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1384711/
Abstract

Human thymocytes bearing the CD45RO 'memory' cell phenotype do not proliferate in concanavalin A (Con A)-stimulated cultures and may be destined for intrathymic death. To determine whether this subset would exhibit characteristics of programmed cell death (apoptosis), we examined the integrity of the nuclear DNA by gel electrophoresis. DNA fragmentation was restricted to the CD45RO+ subset of human thymocytes following exposure to stimulating concentrations of anti-CD2 antibodies. Both CD45RO- and CD45RO+ subsets mobilized cytoplasmic Ca2+ following cell-surface CD2 ligation, but entry into the cell cycle and vigorous thymidine uptake were restricted to the CD45RO- subset. Our results provide a mechanism which may account for the failure of thymic CD45RO+ cells to respond to stimuli which elicit proliferation by the reciprocal CD45RA+ subset.

摘要

具有CD45RO“记忆”细胞表型的人类胸腺细胞在刀豆蛋白A（Con A）刺激的培养物中不增殖，可能注定要在胸腺内死亡。为了确定该亚群是否表现出程序性细胞死亡（凋亡）的特征，我们通过凝胶电泳检查了核DNA的完整性。在暴露于刺激浓度的抗CD2抗体后，DNA片段化仅限于人类胸腺细胞的CD45RO +亚群。细胞表面CD2连接后，CD45RO-和CD45RO +亚群均动员了细胞质Ca2 +，但进入细胞周期和大量摄取胸苷仅限于CD45RO-亚群。我们的结果提供了一种机制，该机制可能解释了胸腺CD45RO +细胞对由相互的CD45RA +亚群引起增殖的刺激无反应的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/1384711/24608cbdc7d8/immunology00109-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/1384711/c61cc8383530/immunology00109-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/1384711/24608cbdc7d8/immunology00109-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/1384711/c61cc8383530/immunology00109-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/1384711/24608cbdc7d8/immunology00109-0101-b.jpg

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1
Differential response of human thymus cells to CD2 antibodies: fragmentation of DNA of CD45RO+ and proliferation of CD45RO- subsets.人类胸腺细胞对CD2抗体的不同反应：CD45RO+亚群的DNA片段化及CD45RO-亚群的增殖。
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引用本文的文献

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PLoS One. 2013 Sep 4;8(9):e73969. doi: 10.1371/journal.pone.0073969. eCollection 2013.
2
Characterization of a monoclonal antibody that recognizes a lymphocyte surface antigen for the cetacean homologue to CD45R.一种识别鲸类动物CD45R同源物淋巴细胞表面抗原的单克隆抗体的特性分析。
Immunology. 1998 Jun;94(2):207-12. doi: 10.1046/j.1365-2567.1998.00483.x.

本文引用的文献

1
Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance.坏死与凋亡：具有根本不同意义的两种不同细胞死亡模式。
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Cell death: the significance of apoptosis.细胞死亡：细胞凋亡的意义
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Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.CD45R缺失和UCHL1反应性增加是预刺激T细胞的一个特征。
J Immunol. 1988 Apr 1;140(7):2171-8.
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Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event.CD45R（Lp220）的缺失代表胸腺后T细胞分化事件。
J Immunol. 1988 Mar 1;140(5):1435-41.
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Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.来自胸腺细胞、T淋巴细胞和B淋巴细胞的大鼠白细胞共同抗原的分子和抗原异质性。
Eur J Immunol. 1985 Feb;15(2):168-73. doi: 10.1002/eji.1830150211.
10
Activation of human thymocytes via the 50KD T11 sheep erythrocyte binding protein induces the expression of interleukin 2 receptors on both T3+ and T3- populations.通过50KD T11绵羊红细胞结合蛋白激活人胸腺细胞可诱导T3 +和T3 -群体上白细胞介素2受体的表达。
J Immunol. 1985 Jan;134(1):330-5.