Plebanski M, Saunders M, Burtles S S, Crowe S, Hooper D C
Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, U.K.
Immunology. 1992 Jan;75(1):86-91.
A culture system has been developed which consistently supports in vitro proliferative responses to conventional soluble antigens by human CD4+ T cells from non-immunized donors. T cells exposed to an antigen in primary cultures could be restimulated in vitro in an antigen-specific manner to give secondary responses with greater magnitudes and a more rapid onset than the initial reaction. To characterize further the responding T-cell population in primary compared with secondary reactions, T cells were depleted of CD45RA+ or CD45RO+ cells and stimulated with recall and non-recall antigens. It was found that the soluble non-recall antigen keyhole limpet haemocyanin did not stimulate CD45RO+ T cells, yet induced strong proliferative responses from CD45RA+ T cells. Conversely, it was confirmed that human CD45RO+ T cells respond to the recall antigen-purified protein derivative from Mycobacterium tuberculosis. Cell mixing experiments indicated that CD45RO+ T cells are unlikely to have any suppressive effect on the reactivity of CD45RA+ cells to non-recall antigens. These data provide new support for the hypothesis that CD45RA+ represents the naive and CD45RO+ the memory phenotype of human CD4+ T cells.
已开发出一种培养系统，该系统能持续支持来自未免疫供体的人CD4 + T细胞对传统可溶性抗原的体外增殖反应。在原代培养中接触抗原的T细胞可在体外以抗原特异性方式再次刺激，从而产生比初始反应强度更大、起始更快的二次反应。为了进一步表征原代反应与二次反应中反应性T细胞群体的特征，去除了T细胞中的CD45RA +或CD45RO +细胞，并用回忆性和非回忆性抗原进行刺激。结果发现，可溶性非回忆性抗原钥孔戚血蓝蛋白不会刺激CD45RO + T细胞，但能诱导CD45RA + T细胞产生强烈的增殖反应。相反，已证实人CD45RO + T细胞对来自结核分枝杆菌的回忆性抗原纯化蛋白衍生物有反应。细胞混合实验表明，CD45RO + T细胞不太可能对CD45RA +细胞对非回忆性抗原的反应性有任何抑制作用。这些数据为CD45RA +代表人类CD4 + T细胞的初始表型而CD45RO +代表记忆表型这一假说提供了新的支持。
