老年人白内障相关的Lewis(x)糖脂在人晶状体中的蓄积。

Senile cataract-related accumulation of Lewis(x) glycolipid in human lens.

作者信息

Ogiso M, Irie A, Kubo H, Hoshi M, Komoto M

机构信息

Department of Physiology and Ophthalmology, Toho University School of Medicine, Tokyo, Japan.

出版信息

J Biol Chem. 1992 Apr 5;267(10):6467-70.

PMID:1348055

Abstract

A glycosphingolipid that reacted positively to anti-stage-specific embryonic antigen-1 (SSEA-1) antiserum accumulated in human lens in association with aging and senile cataract formation. Since this antiserum recognizes Lewis(x) (Le(x)) structure, Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, which is a typical tumor-associated and differentiation-related saccharide chain, the lens glycolipid was predicted to be a Lex antigen. The glycolipid purified from cataractous lens tissues was indeed a Lex glycolipid, Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1- 4Glc beta 1-1 ceramide. Enhanced expression of the Lex glycolipid may affect the organization of lens plasma membranes through Le(x)-Le(x) interactions, as suggested for compaction in mouse preimplantation embryos and embryonic teratocarcinomas, resulting in lens opacification, namely cataract.

摘要

一种与抗阶段特异性胚胎抗原-1（SSEA-1）抗血清呈阳性反应的糖鞘脂，随着衰老和老年性白内障的形成在人晶状体中积累。由于这种抗血清识别Lewis(x)（Le(x)）结构，即Galβ1-4（Fucα1-3）GlcNAc-，这是一种典型的肿瘤相关和分化相关的糖链，因此晶状体糖脂被预测为Lex抗原。从白内障晶状体组织中纯化的糖脂确实是一种Lex糖脂，即Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4Glcβ1-1神经酰胺。Lex糖脂表达的增强可能通过Le(x)-Le(x)相互作用影响晶状体质膜的组织，就像在小鼠植入前胚胎和胚胎性畸胎瘤中的压实作用一样，导致晶状体混浊，即白内障。