Jansen B, Beuth J, Przondo-Mordarska A, Jansen S, Ko H L, Pulverer G
Institute of Medical Microbiology and Hygiene, University of Cologne.
Zentralbl Bakteriol. 1992 Jan;276(2):205-12. doi: 10.1016/s0934-8840(11)80007-1.
Adherence of three fimbriated and non-fimbriated Klebsiella strains to polyetherurethane was investigated in order to assess the possible role of fimbriae in the adhesion of Klebsiella to synthetic polymers. Fimbriated strains with type 1 and type 1.3 fimbriae adhere significantly stronger to polyurethane than the same strains lacking fimbriae, whereas a strain with type 3 fimbriae shows no different adherence compared with the non-fimbriated variant. Analysis of adhesion kinetics and isotherms reveals that adherence of fimbriated Klebsiella strains is proceeded by the formation of multicellular bacterial layers on the polymer surface. Measurements of the relative hydrophobicity of the strains and adherence experiments under exclusion of unspecific interactions point out that fimbriae obviously play a more important role in adhesion than relative hydrophobicity. The demonstration of reduction of bacterial adherence to polyetherurethane by blocking fimbrial action with fimbriae-specific sugars supports this further.
为了评估菌毛在克雷伯菌属与合成聚合物黏附中可能发挥的作用，研究了三种有菌毛和无菌毛的克雷伯菌菌株对聚醚聚氨酯的黏附情况。具有1型和1.3型菌毛的有菌毛菌株比相同的无菌毛菌株对聚氨酯的黏附力明显更强，而具有3型菌毛的菌株与无菌毛变体相比，黏附情况没有差异。黏附动力学和等温线分析表明，有菌毛的克雷伯菌菌株的黏附是通过在聚合物表面形成多细胞细菌层来进行的。菌株相对疏水性的测量以及在排除非特异性相互作用的情况下进行的黏附实验指出，菌毛在黏附中显然比相对疏水性发挥更重要的作用。用菌毛特异性糖阻断菌毛作用可减少细菌对聚醚聚氨酯的黏附，这进一步支持了上述观点。
