Tschäpe H, Bender L, Ott M, Wittig W, Hacker J
BGA, Robert Koch-Institut, Wernigerode.
Zentralbl Bakteriol. 1992 Jan;276(2):264-72. doi: 10.1016/s0934-8840(11)80013-7.
Escherichia coli O139:K82:H1 strains originating from outbreaks and single cases of oedema disease in pigs were characterized by their genomic restriction fragment length polymorphism (RFLP), their virulence pattern, and by the occurrence as well as the genomic distribution of the determinants for hemolysin (hly) and verotoxins (shiga-like toxins; sltI, sltII). Whereas the RFLPs revealed considerable variation among the E. coli O139:K82:H1 isolates depending the origin and epidemic source of the strains, the virulence gene slt II was found to be present in nearly all strains in a particular chromosomal region. Similar to RFLPs, the plasmid profiles are useful for epidemiological analysis.
对源自猪水肿病暴发及散发病例的大肠杆菌O139:K82:H1菌株，通过基因组限制性片段长度多态性（RFLP）、毒力模式以及溶血素（hly）和维罗毒素（志贺样毒素；sltI、sltII）决定簇的出现情况及其基因组分布进行了特征分析。尽管RFLP显示，根据菌株的来源和流行源头，大肠杆菌O139:K82:H1分离株之间存在相当大的差异，但发现毒力基因slt II几乎存在于所有菌株的特定染色体区域。与RFLP相似，质粒图谱对流行病学分析很有用。
