Helgerson Amy F, Sharma Vijay, Dow Amie M, Schroeder Regina, Post Karen, Cornick Nancy A
Department of Veterinary Microbiology, Iowa State University, 2130 Vet. Med. Bldg., National Animal Disease Center, Ames, 50011-1250, USA.
J Clin Microbiol. 2006 Sep;44(9):3074-7. doi: 10.1128/JCM.00617-06.
Edema disease is a systemic disease of weaned pigs caused by host-adapted strains of Escherichia coli, most commonly belonging to serogroup O138, O139, or O141. In the late 1990s, E. coli O147 strains containing the virulence genes f18, sta, stb, and stx(2) were recovered from outbreaks of edema disease in the United States. Pulsed-field gel electrophoresis (PFGE) was used to determine that the majority of these strains (34/43) were closely related to one another. Subsequent analysis by multilocus restriction typing confirmed the PFGE results and indicated that the cluster of edema disease strains were only distantly related to other E. coli O147 strains. Serogrouping of edema disease isolates from the Iowa State University Veterinary Diagnostic laboratory recovered between 1996 and 2000 indicated that 42% belonged to serogroup O147. Our data suggest that these strains may be a common serotype of edema disease-causing E. coli in the United States.
水肿病是一种由宿主适应性大肠杆菌菌株引起的断奶仔猪全身性疾病，这些菌株最常见的属于血清群O138、O139或O141。在20世纪90年代后期，从美国水肿病暴发中分离出含有毒力基因f18、sta、stb和stx(2)的大肠杆菌O147菌株。脉冲场凝胶电泳（PFGE）用于确定这些菌株中的大多数（34/43）彼此密切相关。随后通过多位点限制性分型分析证实了PFGE结果，并表明水肿病菌株簇与其他大肠杆菌O147菌株仅有远缘关系。对1996年至2000年间从爱荷华州立大学兽医诊断实验室分离的水肿病分离株进行血清分型表明，42%属于血清群O147。我们的数据表明，这些菌株可能是美国引起水肿病的大肠杆菌的一种常见血清型。
