Cetron M S, Hoff R, Kahn S, Eisen H, Van Voorhis W C
Department of Medicine, University of Washington, Seattle 98195.
Acta Trop. 1992 Feb;50(3):259-66. doi: 10.1016/0001-706x(92)90082-9.
A perfect serologic test for infection with Trypanosoma cruzi does not exist. This study uses recombinant T. cruzi surface proteins in the antibody capture enzyme linked immunoabsorption assay (ELISA); and compares this approach to the more standard immunofluorescence assay (IFA). Three recombinant antigens are studied: F1-160 corresponding to the 160 kDa flagellar associated surface protein of trypomastigotes (the motile form of T. cruzi in mammalian infections); and SA 85-1.1 and 1.2 corresponding to different members of the 85 kDa family of surface proteins expressed by trypomastigotes and amastigotes (the replicative, non-motile form of T. cruzi in mammalian infections). Each recombinant antigen is found to be highly specific (range 86-94%) but relatively insensitive (range 36-52%) when used to screen for antibodies to T. cruzi. Defining seropositivity as reactivity to any of the three recombinant antigens markedly increases the sensitivity (72%) with only a minor reduction in specificity (82%). Thus, employing recombinant T. cruzi antigens to screen for T. cruzi infection has promise, but improvements in sensitivity must be made before widespread utilization is recommended.
目前尚无用于检测克氏锥虫感染的完美血清学检测方法。本研究在抗体捕获酶联免疫吸附测定（ELISA）中使用重组克氏锥虫表面蛋白，并将该方法与更标准的免疫荧光测定（IFA）进行比较。研究了三种重组抗原：F1 - 160对应于锥鞭毛体160 kDa鞭毛相关表面蛋白（克氏锥虫在哺乳动物感染中的活动形式）；SA 85 - 1.1和1.2对应于由锥鞭毛体和无鞭毛体表达的85 kDa表面蛋白家族的不同成员（克氏锥虫在哺乳动物感染中的复制性、非活动形式）。当用于筛查克氏锥虫抗体时，发现每种重组抗原都具有高度特异性（范围为86 - 94%），但相对不敏感（范围为36 - 52%）。将血清反应阳性定义为对三种重组抗原中的任何一种有反应，可显著提高敏感性（72%），而特异性仅略有降低（82%）。因此，采用重组克氏锥虫抗原筛查克氏锥虫感染具有前景，但在推荐广泛应用之前，必须提高敏感性。
