评估血清学检测以鉴定人类克氏锥虫感染并确定与赖氏锥虫和利什曼原虫属的交叉反应性。
Evaluation of serological tests to identify Trypanosoma cruzi infection in humans and determine cross-reactivity with Trypanosoma rangeli and Leishmania spp.
作者信息
Caballero Zuleima C, Sousa Octavio E, Marques Waldelania P, Saez-Alquezar Amadeo, Umezawa Eufrosina S
机构信息
Instituto de Medicina Tropical de São Paulo and Departamento de Medicina Preventiva da Faculdade de Medicina da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar 470, CEP 05403-000, São Paulo, Brazil.
出版信息
Clin Vaccine Immunol. 2007 Aug;14(8):1045-9. doi: 10.1128/CVI.00127-07. Epub 2007 May 23.
Abstract
Five commercially available enzyme-linked immunosorbent assays (ELISAs), one in-house ELISA, and two hemagglutination assays were evaluated to determine their diagnostic accuracy for Chagas' disease in two studies. In study 1, ELISA kits showed 100% sensitivity, but specificities ranged from 82.84% to 100% when leishmaniasis cases were included and from 95.57% to 100% when leishmaniasis cases were excluded. Kits using recombinant antigens or synthetic peptides are more specific than those using crude extracts from Trypanosoma cruzi epimastigote forms. Kits evaluated in Panama, in study 2, showed 75% to 100% sensitivity and 97.12% to 100% specificity. These data were obtained by using a Western blot assay with T. cruzi trypomastigote excreted-secreted antigens as a reference test to confirm T. cruzi infection.
摘要
在两项研究中，对五种市售酶联免疫吸附测定（ELISA）、一种内部ELISA和两种血凝试验进行了评估，以确定它们对恰加斯病的诊断准确性。在研究1中，ELISA试剂盒显示出100%的灵敏度，但当纳入利什曼病病例时，特异性范围为82.84%至100%，当排除利什曼病病例时，特异性范围为95.57%至100%。使用重组抗原或合成肽的试剂盒比使用克氏锥虫前鞭毛体形式的粗提物的试剂盒更具特异性。在研究2中，在巴拿马评估的试剂盒显示出75%至100%的灵敏度和97.12%至100%的特异性。这些数据是通过使用以克氏锥虫锥鞭毛体排泄-分泌抗原的蛋白质印迹试验作为参考试验来确认克氏锥虫感染而获得的。
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